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A series of 2D 1 H- 15 N HSQC spectra were collected at protein-to-ligand molar ratio of 1:0, 1:0.5, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:5, 1:7, 1:10, 1:15, 1:20, 1:30, 1:40, 1:50 and 1:60 for GlcNAc and 1:0, 1:0.5, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:4, 1:5, 1:6, 1:8, 1:10, 1:15 and 1:20 for GlcNAc1-2Man

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As a result, our finding that small urinary peptides increased in abundance in IC/BPS urine is likely a result of increased proteolysis occurring in the urine or the bladder wall, which is consistent with the inflammatory and immune responses (e.g., mast cell activation) associated with this disorder 1,2,3,4,5,11