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Tests like serum B12 levels and methylmalonic acid (MMA) tests help
Both Dihexa and wortmannin were prepared by dissolving the compounds in 10% DMSA, 40% PEG 300, 5% Tween 80, and 45% saline

At the end of the experiment and after the animals dissection, serum levels of thyroid-stimulating hormone (TSH), cortisol, epinephrine, and vitamin B12 were analyzed
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