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Finally, the bound proteins are eluted by different buffers, containing with either competitive molecules or condition disrupt all interactions
These cytokines launch the inflammatory cascade by activating transcription factors like NF-B, which subsequently upregulate the expression of other cytokines, chemokines, and adhesion molecules
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Outside of the liver, this pathway is absent or insignificant, either in normal or transformed tissues (Finkelstein, 1990)
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