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Cells were harvested, washed once with phosphate buffered saline (PBS, Gibco, #14190-094) and loosened from the cell flasks with Versene (Gibco, #15040-033) or TrypLE TM (Gibco, #12605-010)

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When performed by a trained provider using medical-grade product, correct dosing and sterile technique, GHK-Cu injections are generally well tolerated

[DOI] [PubMed] [Google Scholar] 170.Edenberg HJ, Xuei X, Chen HJ, Tian H, Wetherill LF, Dick DM, Almasy L, Bierut L, Bucholz KK, Goate A, et al