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In vitro expansion and genetic modification of gastrointestinal stem cells in spheroid culture

GPOX (EC 1.11.1.7) activity was assessed by examining the conversion of 0.1 mM guaiacol in the presence of 1 mM H 2 O 2 , with the progress monitored through the formation of tetraguaiacol observed at 470 nm

The two main sources of intracellular Fe 2 + accumulation during ferroptosis are: (1) TF binds to TFR and enters the cell, where the low pH value promotes the release of Fe3 + from TF, which is then reduced to Fe 2 + in the cytoplasm

Published microarray analyses have reported effects on genes involved in anti-inflammatory responses, antioxidant defense systems, stem cell markers, and DNA repair pathways

Amaro S, Jimnez-Altay F, Chamorro