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Protein lysates were boiled at 95 C for 10 min on a thermomixer (Thermo Fisher Scientific) and sonicated on the Bioruptor (Diagenode) system

Proteins were injected intraperitoneally at the time of inoculation with 20 mg kg 1 cetuximab (3.33 nmol per animal), 2.2 mg kg 1 Albu-LiTE construct (500 pmol per animal) or 1.0 mg kg 1 LiTE (500 pmol per animal)

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In a previous comprehensive review about protein carbonylation in meat systems, Estvez (2011) already warned that most causative connections between protein carbonyls and the alleged effects on protein functionality were based on the calculations of positive and significant correlations