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2.7 Aortic ring assay The mouse aortic ring assay was conducted according to a previously described method ( 2.8 Western blotting Proteins were extracted from the tissue samples, C2C12 myocytes, or HUVECs using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Nacalai Tesque, Kyoto, Japan), separated using SDS-PAGE, and transferred to PVDF membranes

Need ultrasonic Purity & Documentation Data Sheet (272 KB) SDS (394 KB) English - EN (394 KB) Franais - FR (394 KB) Deutsch - DE (394 KB) Norwegian - NO (394 KB) Espaol - ES (394 KB) Swedish - SV (394 KB) Italian - IT (394 KB) Korean - KR (394 KB) Portuguese - PT (394 KB) Handling Instructions (2659 KB) References [1]