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This method can detect as low as 17.5 10 5 EVs/L (Kalimuthu et al., 2019)

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Necrotic and apoptotic signalling in mouse myocardial I/R injury Troponin in serum To examine necrotic cell death, blood was collected from the abdominal aorta after 24 h of reperfusion following 30 min of ischaemia
