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doi: 10.1016/j.devcel.2016.03.015 83 OkudaKSMisaJPOehlersSHHallCJEllettFAlasmariSet al
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After three washes (20 min each) with Tris-buffered saline containing 0.1% Tween 20, the membrane was incubated (1 h) with peroxidase-conjugated secondary goat anti-mouse (1:5000) or anti-rabbit (1:5000) and washed again three times
