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Briefly, for RP-HPLC analysis, an UltiMate 3000 HPLC from Thermofisher with a Fortis Bio C18, 3 m, 4.6 100 mm reversed-phase column was used and analysed using the Chromeleon software (v6.8)

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After staining, cells were fixed in 4% PFA, dried onto glass slides and examined by epifluorescence microscopy (Olympus, BX60)

Studies in humans probing association between postnatal exposure to APAP and ASD Limited studies have attempted to ascertain the association between postnatal exposure to APAP and ASD in humans