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Nuclei were isolated from frozen, ground plant tissue using ice-cold Honda buffer (0.44 M sucrose, 1.25% (w/v) Ficoll, 2.5% (w/v) dextran T40, 20 mM HEPES-KOH pH 7.4, 10 mM MgCl 2 , 0.5% (w/v) Triton X-100, 1 mM DTT, 1 protease inhibitor (Roche) and 0.4 U l 1 Ribolock RNase inhibitor (Thermo Fisher Scientific)) and collected via centrifugation at 2,000 g for 5 min at 4 C

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used DIOS technology to achieve cellular-level imaging of small molecules in biological tissues on porous silicon substrates

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Regular skin examinations and timely evaluation of suspicious lesions play a critical role in early detection