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This is because the vast majority of harmful chemicals found in cigarette smoke and linked to smoking-related diseases are generated by combustion

The results were published in Nature on Dec 7

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After incubation with blocking buffer (PBS-C/M containing 0.5% BSA, 0.5% Triton X-100, and 0.2 mg/ml sodium azide) for 30 min at room temperature, the membranes were incubated with anti-ZO-1 antibody in PBS-C/M for 1 h at room temperature

Heidi Green, MD Medical Reviewer Chris Iliades, MD Author Chris Iliades, MD, is a full-time medical writer and journalist based in Boothbay Harbor, Maine