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Conventional and microwave-assisted fixation substitution, resin embedding, sectioning, and microscopic analysis were performed as described ( In Situ Detection of Reactive Oxygen Species Reactive oxygen species cellular localization was determined by confocal microscopy in an Eclipse TE2000E2 Confocal Laser Scanning Microscope (Nikon) with excitation at 488 nm and emission at 515/530 nm, after leaf staining with the ROS-sensitive fluorescent probe DCFDA

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6/11/2022 - HomeAdvisor Jack S