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AHM and CJLM designed the overall study and analytical strategy

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A total of 1.2 g of RNA was utilized for reverse transcription, accomplished using the MMLV Reverse Transcriptase kit (M1701, Promega, Walldorf, Germany), Random Hexamer Primers (48,190,011, Thermo Fisher), and RiboLock RNase Inhibitor (EO0384, Thermo Fisher)

These statistics are especially unfortunate in that smoking is considered the leading cause of preventable deaths in the United States

Its usually done by a machine, so its very reflective