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30 Nicotine is metabolized primarily to cotinine, which is further metabolized to trans-3-hydroxycotinine (3HC), catalyzed by the liver cytochrome P450 2A6 enzyme

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References 1 BMC Medicine: Relationship of tobacco smoking to cause-specific mortality: contemporary estimates from Australia 2 The Sax Institutes 45 and Up Study: www.saxinstitute.org.au/solutions/45-and-up-study/