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As the brain does not reach full development until the age of 25 years, nicotine use by those aged 1824 years continues to make detrimental changes to their brain functionality, contributing to addiction susceptibility 1,6

Reference Dobrinas, Choong, Noetzli, Cornuz, Ansermot and Eap2011) used a 0.5mLmin 1 flow rate

9 Evidence of the harm caused by passive smoking inspired the non-smokers rights movement, which largely bypassed the Congress and focused political pressure on state and local governments

Generative AI statement The authors declare that no Generative AI was used in the creation of this manuscript

The SA standard was diluted in methanol to prepare a series dilutions of SA at the concentrations of 3.125, 6.25, 12.5, 25, 50 and 100 g/mL, and the absorbance at 297 nm was used to construct the standard curve