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Tobacco use encompasses smoking cigarettes, cigars, or pipes, as well as consuming smokeless tobacco products

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Endogenous Viral Element (EVE)-Derived siRNAs Besides LTR retrotransposons of the families Metaviridae (Ty3/Gypsy, ssRNA-RT) and Pseudoviridae (Ty1/Copia, ssRNA-RT) that spawn 21-nt, 22-nt, and 24-nt siRNAs as discussed above, EVEs of the families Caulimoviridae (dsDNA-RT), Geminiviridae (ssDNA) and Bromoviridae [(+)ssRNA] have been characterized by small RNA sequencing (see Supplementary Table S1 and references therein)

The plant contains two alkaloid chemicals