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Hebels DG, Bried JJ, Khampang R, et al

Dimethyl sulfoxide (DMSO), agar, glucose, 2-aminoanthracene(2-AA), L-biotin, L-histidine, citric acid monohydrate, potassium phosphate dibasic anhydrous (K 2 HPO 4 ), crystal violet, magnesium sulfate (MgSO 4 7H 2 O), sodium ammonium hydrogen phosphate (NaNH 4 HPO 4 4H 2 O), and ampicillin were purchased from Sigma Co (St Louis, USA), S-9 (protein content : 23.6 mg/mL) was purchased from Moltox Co (USA) and S-9 cofactor was purchased from Oriental Yeast Co (Japan)