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However, epidemiological studies assessing Swedish smokeless tobacco, which exposes users to nicotine without combustion products, generally have not found an increased risk of myocardial infarction or stroke among users, but suggest that nicotine might contribute to acute cardiovascular events, especially in those with underlying coronary heart disease

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The slides were then rinsed with PBS buffer and mounted with a 1:1 (v/v) PBS/glycerol solution before being stored at 4 C overnight