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Nonspecific [ 35 S]GTPS binding was determined in the presence of unlabeled GTPS (10 M)

Numerous studies indicated that smoking had adverse effects on human health and represented a predisposing factor for development of various pathological conditions and diseases, such as the chronic obstructive pulmonary disease (1), cancer (2), pancreatitis (3), gastrointestinal disorders (4), periodontal disease (5), metabolic syndrome (6), and some autoimmune diseases (7)

Histone H3 (4499, Cell Signaling, RRID: AB_10544537) was used as a nuclear fraction loading control, and -tubulin (ab7291, Abcam) as the cytosolic fraction loading control

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