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Passage 1 or 2 cultures were seeded onto collagen IV (C7521, Sigma, St Louis, MO) coated 12 mm Transwell inserts (3460, Corning Costar Corporation, Cambridge, MA) and grown in ALI media to 80% confluence before exposing the apical surface to air

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Smoking Heaviness A total of 11 studies investigated the association of baseline depression with subsequent heaviness of smoking, of which eight (73%) found evidence that depression was associated with heavier rates of smoking, 22,119125 while two (18%) found that depression was associated with reduced heaviness of smoking 26,126 and one (09%) found no evidence of an association

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