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doi: 10.1186/s12931-023-02662-5 11

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The PM investigators [18] reported a few minor differences in the biological activity of the rats exposed to cigarettes with the additives, but dismissed these differences as following no clear pattern, suggesting they are due to statistical inference. The overall conclusion, reported in the paper's abstract is that the data [from a 90-d inhalation study] indicate that the addition of the 333 commonly used ingredients, added to cigarettes in three groups, did not increase the inhalation toxicity of the smoke. The animal toxicology results [18] reported from Project MIX were based on a small number of rats in each experiment (usually nine), raising the possibility that the failure to detect statistically significant changes in the end points were due to underpowering the experiments rather than lack of a real effect