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Immunohistochemistry was performed as follows: free-floating VTA brain sections were incubated 1 h at 4 C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma A4503) and 0.2% Triton X-100 and then incubated overnight at 4 C with a mouse anti-tyrosine hydroxylase antibody (TH, Sigma, T1299) at 1:500 dilution in PBS containing 1.5% BSA and 0.2% Triton X-100 (see supplementary Table 1)

moderate-quality evidence), respectively

CA: A Cancer Journal for Clinicians 2006
First, COPD patients have increased NKp44 ILC3(NCR ILC3) frequencies in their blood compared to control groups 27,28

N Engl J Med 332 , 11981203, (1995)
