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Furthermore, while the application of CRISPR/Cas9 is limited by its requirement for a specific protospacer adjacent motif (PAM) sequence featured by a 5NGG3 pattern, the discovery of Cas variants such as Cas12a (also known as Cpf1), which recognize a distinct 5TTTN3 PAM sequence, has significantly broadened the scope and versatility of genomeediting applications (Gao et al., 2017)

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