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Other evidence of the possible irritant and inflammatory effect on cells comes from an in vitro study in which the exposure of tracheobronchial cells to electronic cigarette nicotine-free vapours was related to a concomitant increase in interleukin-6 and interleukin-8 cytokine production with a dose-dependent response and direct evidence of endothelial dysfunction.84 The association with inflammation and endothelial dysfunction is most likely mediated via nitric oxide pathways

38.Miller V, Reynolds RJ

By World Health Organization region, nine of the studies were from Europe (Denmark, France, Spain and the UK), 17 from the Americas (Brazil, Canada, Haiti and the US), seven from Africa (Rwanda, South Africa, Tanzania, Uganda and Zimbabwe), two from the western Pacific (Australia and China) and just one from south-east Asia (India)