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Until the advent of modern pesticides, farmers would examine each plant in a field and remove the worms by hand

Indeed, while nicotine in normal doses is not as dangerous as perceived, the Surgeon Generals report in 2014, The Health Consequences of Smoking50 Years of Progress, surmised that nicotine has acute toxicity at high doses and activates biological pathways that upsurge the risk of disease

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The fungal ITS1-5F region was amplified using the primers ITS1F (5-CTTGGTCATTTAGAGGAAGTAA-3) and ITS2R (5-GCTGCGTTCTTCATCGATGC-3) (Xiang et al., 2020a)