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Flow cytometry For the flow cytometric analysis of peripheral blood, whole blood was drawn by retro-orbital sinus puncture, treated with RBC lysis buffer, stained with antibodies (APC-CD45, PE-anti-GL3, FITC-Ly6G, and Percp-cy5.5-CD11b), gated for singlet cells, and then plotted on a dot-plot of different groups

Drug Enforcement Administration ranks controlled substances according to five schedules. Marijuana, along with drugs like heroin and LSD, is a Schedule I drug, meaning it is considered to have the highest potential for abuse and be the most dangerous. Because alcohol and tobacco are legal, they are not controlled substances, and not subject to DEA scheduling