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Involvement of P53, BCL-XL, AIF and autophagy in CSC-induced cell death: differences between lower and high CSC concentrations To further analyse the involvement of P53 and the anti-apoptotic mitochondrial protein BCL-XL in CSC-induced cell death, we infected endothelial cells with retroviruses containing a P53 shRNA vector and as P53-activated death signalling is known to be mediated through pro-apoptotic BCL-2 family members we generated human umbilical vein endothelial cells (HUVECs) overexpressing the anti-apoptotic protein BCL-XL