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A549, RPMI2650, BEAS-2B, or MLE12 cells were seeded on 12 well-transwell inserts (Corning, ME, USA) for 24 h before CSE treatment and then, cells were cultured with 10% CSE-containing media for 48 h, rinsed with PBS

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Use a sponge or spray bottle to lightly apply the solution, then blot with a soft cloth

He acquired a reputation for smoking cigars and cigarettes during rounds almost rivaling that of John Daly, a contemporary