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46, 56, 61 In addition, cigarette smoke extract induced monocyte chemotactic activity by lung epithelial cells and fibroblasts in vitro through the release of CCL2

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We carried out the integration analysis using the top 30 dimensions from a canonical correlation analysis (CCA) based on SCTransform normalized expression of the top 3000 most informative genes across donor datasets, where informativeness was defined by gene dispersion (i.e., the log of the ratio of expression variance to its mean) across cells, calculated after accounting for its relationship with mean expression (using the SelectIntegrationFeatures function)