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Briefly, whole blood samples (0.5 mL) from each subject were cultured in 4.5 mL RPMI-1640 medium with L- Glutamate (Gibco-Invitrogen) supplemented with Fetal Bovine Serum (25%, Sigma), phytohemaglutinin (2.5%, Gibco-Invitrogen), Penicillin-Streptomycin (1.5% Gibco-Invitrogen) and sodium heparin (0.5%, B

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