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Passage 1 or 2 cultures were seeded onto collagen IV (C7521, Sigma, St Louis, MO) coated 12 mm Transwell inserts (3460, Corning Costar Corporation, Cambridge, MA) and grown in ALI media to 80% confluence before exposing the apical surface to air

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Except in this directors imagination, Micaela, an innocent and unsuspecting lass, creeps back in the shadows to observe Joss first fateful dalliance with Carmen

This emulation speaks to the enduring influence and impact of the cigarette boat design, further cementing its status as an icon in the world of powerboating