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hLMVECs were first seeded on the basolateral side of each membrane in the AX12 at a density of 106,000cells/cm 2 , followed by a 2-h incubation, and then AX iAECs were seeded apically at a density of 409,000 cells/cm 2 on day 0 for all co-culture experiments

The beds were slept in, played on, fought over, scratched up, and generally used (or ignored) by a total of 25 cats, ranging in age from 11 months to 16 years