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Cigarettes were touted as risk-free, and even doctors endorsed them as safe
The cp gene was amplified from vector pET22b-TMV_CP (unpublished data) using primers Len-CP U1_fw/Len-CP U1_rev (Supplementary Table S1) to facilitate Gibson assembly (NEB, Frankfurt am Main, Germany)
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Southern states broke with the union that had been formed after the Revolution, declaring themselves a separate entity, the Confederate States of America
