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This increase includes all tobacco products, including vapes, e-cigarettes and chewing tobacco
Furthermore, most of the metabolites (14/22) produced from nicotinate and nicotinamide metabolism, tryptophan metabolism, tyrosine metabolism, and phenylalanine metabolism (Figure 6) showed an increasing trend in content during late-stage fermentation (T2, T3), further aligning with Liu et al
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Finally, thin the seedlings out by removing all but the best one or two plants per pot, eventually only leaving one plant

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