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Description
Passage 1 or 2 cultures were seeded onto collagen IV (C7521, Sigma, St Louis, MO) coated 12 mm Transwell inserts (3460, Corning Costar Corporation, Cambridge, MA) and grown in ALI media to 80% confluence before exposing the apical surface to air
To avoid potential complications, such as infection, its important to understand how to treat these skin injuries
"Sales picked up enormously." But, he warns, green may be ugly, but not always off-putting
Dnmt3L cooperates with the Dnmt3 family of de novo DNA methyltransferases to establish maternal imprints in mice
The primer sequences are provided in supplementary Table 1
