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One group of investigators used a vibrational nebulizer to expose C57BL/6 and CD-1 (outbred) mice to 1 h of e-cig aerosol (25 mg/ml nicotine) three times over 24 h (115)

To avoid inhalation of vapors created during spraying or direct contact with applied substances, it is recommended to wear complete protective equipment

The most common type of cigarette pack is the rigid pack in the American market

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An independent study found higher expressions of metallothionein (scavengers of ROS and metals and associated with immune diseases and cancers) in the cells of the lungs and liver from mice exposed to CS but not to IQOS smoke 33