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Lastly, aqueous extracts are not suitable to ALI cell models since the application of these extracts is based on cell submersion, a non-physiological exposure method that annihilates the benefits of ALI

What are the respiratory effects of e-cigarettes

2018;16: e2003904

It is difficult to know exactly what is found in e-cigarettes as there have been some marketed as containing zero percent nicotine when in fact they were tested and did contain nicotine

The huge variation in study designs assessing in vitro toxicity of e-cigarettes aerosol makes it difficult to make comparisons and draw robust and irrefutable conclusions