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Cloning and mutagenesis Human NLRP3 cDNA was cloned into a pcDNA3.1D/V5-His vector provided in the pcDNA3.1D TOPO cloning kit

1-antitrypsin deficiency: A misfolded secretory protein variant with unique effects on the endoplasmic reticulum

S., Stanfill, S

The E-cig group continued exposure at a reduced duration of 1 h/day to simulate gradual tapering
