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these cells subsequently enter the arterial wall and promote atherosclerosis.95 In young adults who chronically used electronic cigarettes, FDG-PET CT showed increased uptake in the spleen and aortic wall compared with non-users, which is consistent with the engagement of the splenocardiac axis by the substances released by these devices.96 The effect on physical properties of the cardiovascular system come from a study of mice exposed to electronic cigarette vapours for 8 months (4 hours a day for 5 days a week, emulating an equivalent of 20 years of exposure in humans)
Clair C, Cohen MJ, Eichler F, Selby KJ, Rigotti NA

2017;358:j3262

Studies using salivary cotinine found that a significant proportion of blunt smokers who reported no tobacco use still showed measurable nicotine exposure, confirming the outer leaf transfers nicotine during smoking tobacco-wrapped cannabis
