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The researchers used gas and liquid chromatography to determine what was in the vapor, looking at the first puffs as well as later puffs after the device heated up and reached a steady state. Not all puffs are equal One finding was that the first and last puffs produce widely varying emissions

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Such as carbon monoxide, nitrogen oxides, and other harmful gases, as well as hydrogen cyanide and other volatile organic compounds

Drawing on data from 977 individuals who reported past-year ENDS use at age 23, the researchers used logistic regression analysis to determine whether reasons for ENDS use at age 23 was associated with ENDS/cigarette use at age 26