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71 DIAGNOSIS AND DIFFERENTIAL DIAGNOSIS The diagnosis of myasthenia is usually established clinically and supported by a positive response to one or more of the following tests: serologicalautoantibodies against the AChR or MuSK pharmacologicalresponse to edrophonium electrophysiologicalrepetitive nerve stimulation (RNS) or single-fiber electromyography (SFEMG) As has been repeatedly emphasized, MG should be considered in any patient with painless weakness, occurring in a regional, multifocal, diffuse, or even a seemingly focal pattern