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Description
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42 While a severe loss of activity (10000-fold) was reported when it was substituted for Ala, 29 replacement with Glu led to only a moderate loss in affinity (31-fold), confirming the requirement of a negatively charged amino acid at position 15
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MN patches were affixed to the top of the aluminum block with a thin layer of Loctite 411 adhesive (Henkel, Germany)
