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When designing a neo agonist, the length of the C-terminal may not be as important as previously thought

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Abbreviations AD Alzheimer's disease AGBL4 ATP/GTP binding protein like 4 BAX Bcl-2-like protein 4 Bcl2 B-cell lymphoma 2 BDNF Brain-derived neurotrophic factor CNS Central nervous system CREB CAMP response element-binding protein DMEM Dulbecco's modified eagle medium FBS Fetal bovine serum GAP43 Growth associated protein 43 GIP Glucose-dependent insulinotropic polypeptide GLP1-RAs Glucagon-like peptide 1 receptor agonists GLUT1 Glucose transporter 1 GLUT3 Glucose transporter 3 GLUT4 Glucose transporter 4 HG High glucose Ki-67 Antigen Kiel 67 MAP2 Microtubule-associated protein 2 NG Normal glucose pAkt Phospho protein kinase B PBS Phosphate-buffered saline SHSY5Y Neuroblastoma cell line SORBS1 Sorbin and SH3 containing domain T2DM Type 2 diabetes mellitus TIR Tirzepatide TrisHCl Tris hydrochloride NaCl Sodium chloride NaF Sodium fluoride PMSF Phenylmethylsulfonyl fluoride SDS-PAGE Sodium dodecyl sulphatepolyacrylamide gel electrophoresis TBS Tris-buffered saline Author contributions RAF and PG performed the experiments and data analysis, wrote the manuscript