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Blood and tissue collection Blood was taken at the start of the light cycle in the ad libitum state at weeks 1, 2 and 4 in the Glp2r / mice and WT controls, where 10% TED (5000 kIU/mL Trasylol, 1.2 mg/mL ethylenediaminetetraacetic acid (EDTA), 0.1 nmol/L Diprotin A) (vol/vol) was added for active plasma GLP-1 (K1503OD-2, MSD) measurements

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This gradual increase helps to lower side effects like nausea