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After completion, as monitored by UPLC and ESI-LCMS, the reaction mixture was diluted with buffer A, filtered and purified directly by preparative RP-HPLC, eluting with a linear gradient (see the Peptide cleavage and purification section)

Peptide samples with ThT were pipetted into a 96-well half a rea plate (Corning 3881) and sealed with tape (Costar Thermowell) to prevent the samples from evaporating