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Statistical analysis For animal studies, sample sizes ( n = 5 per group, 3 in the tirzepatide-treated group) were determined based on prior in-house experience and previous studies using similar mouse models to ensure adequate power to detect biologically relevant effects

In the SURPASS-2 trial, Tirzepatide participants experienced substantial reductions in HbA1c levels and body weight compared to those treated with semaglutide

The baseline characteristics by status of prediabetes or diabetes revealed similar distributions as Table 1 (Table S6)

Still, studies are small and not very conclusive in humans

Each blood sample was about 200 L, resulting in about 100 L plasma