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Stable CHO-SNAP-GLP-1R and MIN6B1-SNAP-GLP-1R cells were generated by transfecting pSNAP-GLP-1R (Cisbio) into wild-type CHO-K1 (ECACC) or MIN6B1, G418 (1 mg ml 1 ) selection, and single-cell sorting by fluorescence-activated cell sorting (FACS) following SNAP-Surface-488 (New England Biolabs) labeling

It involves removing a portion of the stomach and rerouting the small intestine to reduce the absorption of calories and nutrients

At 24 weeks, a statistically significant reduction in A1C occurred in all treatment groups ( P .0001), compared to the metformin plus placebo group

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Cellular bioenergetic parameters including ATP- and non-ATP-linked oxygen consumption as well as maximal oxygen consumption and reserve capacity were calculated (Figure 1)