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Description
Following immunoprecipitation, the bead-bound SNAP/FLAG-hGLP-1R was incubated with a click chemistry mix (20 M rhodamine-azide, 1 mM TCEP, 100 M TBTA, 1 mM CuSO 4 ) for 1 hour at room temperature gently agitating to fluorescently label the PhotoClick cholesterol
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Biol Psychiatry (1987) 22:7989.10.1016/0006-3223(87)90219-8 89 SaranAS
