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Cells or islets were incubated for 24 hours post-transfection / transduction before labelling with 1 M SNAP-Surface fluorescent probes (New England Biolabs) for 15-20 minutes for cells, or 7 minutes for islets, in full media at 37C

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Part 1 of 2 covers the mechanism and the U/kg trend, the portal-peripheral mismatch, and the Ominous Octet framework

[34] [35] In the brain, they act on satiety centers to reduce appetite and food intake